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This can be especially real for immunohistochemistry during which 10% NBF has become demonstrated to be a weak fixative (Fig. 2). Some issues with the use of 10% NBF like a fixative are corrected with the introduction of other experimental tactics for example antigen retrieval (Shi et al. 2001) and using tiny amplicons in RT-Q-PCR (Steg et al. 2006

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